Hong-Ling LIU 刘虹伶, Yan-Ping WANG 王燕平, Song CHEN 陈松, Cui-Cui ZHANG 张翠翠,

Qing-Dong CHEN 陈庆东, Chun-Yan YI 易春燕, Xu LIU 刘旭 & Mao WANG 王毛*

Abstract: Diaphorina citri Kuwayama (1908), the Asian citrus psyllid (ACP), is a major pest of citrus crops, responsible for transmitting the bacterium that causes Huanglongbing (HLB), a disease devastating to citrus industries worldwide. Despite extensive research on its population genetics in other regions, the genetic structure of D. citri in Sichuan Province, China, remains poorly understood, complicating the development of effective pest management strategies. This study aims to fill this gap by investigating the genetic diversity and population structure of D. citri using genome-wide single nucleotide polymorphisms (SNPs). Three natural populations of D. citri from southeastern Sichuan were sequenced, revealing a total of 7,010,305 SNPs and relatively low genetic differentiation among populations. Phylogenetic analysis identified two primary genetic groups with limited gene flow, supported by principal component analysis (PCA) and Admixture analysis. The populations exhibited high genetic diversity, with notable variations in nucleotide diversity and inbreeding coefficients. Functional enrichment analysis highlighted genes involved in cellular processes and stress response, which may contribute to local adaptation. These findings provide valuable insights into the evolutionary dynamics of D. citri and suggest that genetic diversity and selective pressures play a key role in its adaptability. The results underscore the importance of considering genetic variation in the development of targeted and sustainable pest management strategies in southeastern China.

Keywords: Genetic differentiation, Phylogeography, Population genetics, Population history, Whole-genome sequencing

摘要：柑橘木虱Diaphorina citri Kuwayama, 1908是柑橘作物的主要害虫，负责传播引起黄龙病（Huanglongbing, HLB）的病原细菌，该病对全球柑橘产业造成毁灭性打击。尽管在其他区域对其种群遗传学已有广泛研究，但中国四川省柑橘木虱的遗传结构仍知之甚少，这阻碍了有效害虫管理策略的制定。本研究旨在利用全基因组单核苷酸多态性（SNPs）技术，调查四川省柑橘木虱的遗传多样性与种群结构，以填补这一空白。对来自四川省东南部的三个自然种群进行了测序，共检测到7,010,305个SNPs，种群间遗传分化程度相对较低。系统发育分析、主成分分析（PCA）和Admixture分析支持存在两个主要的遗传类群，且基因流有限。种群表现出较高的遗传多样性，核苷酸多样性和近交系数存在显著变异。功能富集分析强调了涉及细胞过程和胁迫响应的基因，这些基因可能有助于本地适应。这些发现为理解柑橘木虱的进化动态提供了有价值的见解，表明遗传多样性和选择压力在其适应性中起着关键作用。研究结果强调了在制定中国东南部靶向和可持续害虫管理策略时，考虑遗传变异的重要性。

关键词：遗传分化，系统地理学，群体遗传学，种群历史，全基因组测序

Published online: 2025-09-04 12:37:40

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